Integrated Transcriptomic and Targeted Metabolomic Analysis Reveals Regulation of Carotenoid Accumulation During Pepper Fruit Development
In this study,the fruit of pepper inbred line 1189 during different developmental stages was used to investigate the profiles of carotenoids by combination of metabolomic and transcriptomic analyses.Transcription factors that may be involved in regulating carotenoid biosynthesis in pepper were identified by weighted gene co-expression network analysis(WGCNA).A total of 60 carotenoids were identified by targeted metabolome.After analysis,it was found that the main components at young fruit stage and mature green fruit stage were lutein(73.4％and 64.5％)and the lutein contents sharply decreased at color-changed fruit stage.The capsanthin content(32.5％)was highest at red ripe fruit stage and the lutein could not be detected.The 14 148 differentially expressed genes(DEGs)and 15 DEGs related to carotenoid metabolism were identified respectively by transcriptomic.The 12 286 DEGs screened were divided into 16 modules by WGCNA.It was found that MEdarkseagreen4,MElightcyan1 and MEbrown2 module were significantly correlated with 11 major carotenoids.The metabolic regulatory network was constructed based on carotenoid-related DEGs in the three modules,and ten transcription factors,including bHLH48,SEP3,CMB1,AGL27,YABBY2,GT-3B,NF-YA3,NAC2,HOX5 and GATA26,were obtained through promoter binding motif analysis,which may play important regulatory roles in carotenoid accumulation.
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