Molecular Cloning and Functional Analysis of AcGAI in Onion Flowering Regulation
AcGAI of DELLA family was cloned from a long-day ecotype onion inbred line SB2.Sequence analysis showed that the full length of AcGAI coding region was 1 575 bp,which was estimated to encode 524 amino acids.AcGAI has typical DELLA and GRAS domain.qRT-PCR results showed that AcGAI was expressed in the leaves,leaf sheaths,bulbs,flower stems and flowers of onion during the reproductive growth stage,and the expression level was higher in the flowers.AcGAI overexpressed Arabidopsis thaliana transgenic lines showed delayed flowering time.AcGAI was heterogeneously overexpressed in the gai and della mutants of Arabidopsis thaliana,and the early flowering phenotypes of the gai and della mutants were inhibited to varying degrees.qRT-PCR was used to detect the expressions of the photoperiodic core gene CO and the flowering regulation key gene FT in AcGAI overexpressed plants,and it was found that the expressions of CO and FT were significantly decreased.AcGAI interact with AcCO by yeast two-hybrid system and bimolecular fluorescence complementation technique.This study suggests that GAI involved in flowering regulation by regulating the expression of CO.
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