Identification and Expression Pattern Analysis of GST in Zingiber officinale
To analyze the information of the glutathione-S-transferase(GST)gene family in ginger(Zingiber officinale Roscoe.),based on ginger genome data,the members and expression patterns of the ZoGST family were identified and analyzed.Furthermore,the expression characteristics of 10 ZoGST under different stress conditions were detected using qRT-PCR.A total of 58 members of the GST family were identified in the ginger genome,encoding proteins with amino acid lengths ranging from 110 to 675 aa,molecular weights ranging from 12.21 to 72.51 kD,isoelectric points ranging from 4.55 to 9.51.The phylogenetic tree shows that ZoGSTcan be divided into Ⅰ-Ⅹ subfamilies,with members from the Ⅶ and Ⅹ subfamilies being the majority.The members of the ZoGST family are distributed on 11 chromosomes,and collinearity analysis shows that there are 6 pairs of segment duplications in this family member.Analysis of cis-acting regulatory elements revealed that the upstream promoter region contains regulatory elements related to light response,stress,and hormone regulation.Transcriptome sequencing data analysis shows that ZoGST has certain tissue expression specificity and responds to stress such as diseases and low temperatures.Among them,ZoGSTDHAR5,ZoGSTF9,ZoGSTF13,etc.are highly expressed in ginger at different growth stages,different parts,low temperatures,and disease stresses.qRT-PCR analysis showed that under salt stress,the expression levels of ZoGSTDHAR2(except for rhizomes),ZoGSTEF1G2,and ZoGSTF9 were significantly downregulated in different tissues of ginger.Under flooding stress,the expression levels of ZoGSTDHAR2 and others were significantly upregulated in leaves and rhizome.Under drought stress,the expression levels of ZoGSTF9 and others were significantly upregulated in roots.
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