首页|云南大花蕙兰复合感染的ORSV和CyCMV病毒基因组序列特征分析

云南大花蕙兰复合感染的ORSV和CyCMV病毒基因组序列特征分析

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从云南丽江采集了40个大花蕙兰(Cymbidium hybridum)感病样品，利用高通量转录组测序对其中的病毒种类进行分析，Blastn N比对发现样品中存在建兰花叶病毒(cymbidium mosaic virus，CymMV)、齿兰环斑病毒(odontoglossum ringspot virus，ORSV)和建兰褪绿花叶病毒(cymbidium chlorotic mosaic virus，CyCMV)。利用RT-PCR对样品中病毒分离物的CP序列进行扩增，10份样品为ORSV和CyCMV复合侵染。为进一步明确ORSV和CyCMV云南分离物(ORSV-DH和CyCMV-DH)的序列特征，分别对其全基因组序列进行了扩增及克隆，结果表明:ORSV-DH基因组全长序列为6 611 nt(Accession No。OP644547)，CyCMV-DH 基因组全长序列为 4 083 nt(Accession NO。OP629812)，将 ORSV-DH 和CyCMV-DH分别与己报道的分离物的全基因组序列进行核苷酸序列分析，发现ORSV-DH与其他分离物的核苷酸相似性为97。69％～99。41％，CyCMV-DH为89。84％～98。19％。根据病毒全基因组核苷酸序列分别构建了ORSV-DH和CyCMV-DH与本属其他分离物的系统进化树，明确了其分别与日本分离物ORSV-Cy-1(S83257)、CyCMV-Japan(NC_027123)亲缘关系最近。
Molecular Characterization of the Odontoglossum Ringspot Virus and Cymbidium Chlorotic Mosaic Virus Mix-Infecting Cymbidium hybridum in Yunnan
Forty greenhouse-grown orchid samples were collected in Lijiang City,Yunnan Province,China,to assess the presence of viruses.Total RNAs from two samples were extracted and subjected them to high-throughput transcriptome sequencing(HTS)using the Illumina HiSeq platform.BLASTN searches revealed the presence of contigs with high similarities to cymbidium mosaic virus(CymMV),odontoglossum ringspot virus(ORSV),and cymbidium chlorotic mosaic virus(CyCMV).RT-PCR assays were conducted to confirm the presence of these viruses in the individual collected samples,and 10 samples were found to be mix-infected by ORSV and CyCMV.To further ascertain their molecular characteristics,the complete genomic sequences of the ORSV-DH and CyCMV-DH isolates were cloned.The full-length sequences of the ORSV-DH and CyCMV-DH genomes were determined to be 6 611 nt(GeneBank accession No.OP644547)and 4 083 nt(GeneBank accession No.OP629812),respectively.Sequence alignments revealed that ORSV-DH displayed nucleotide sequence identities of 97.69％-99.41％with previously reported ORSV isolates,whereas CyCMV-DH showed identities of 89.84％-98.19％with other CyCMV isolates.Phylogenetic trees of ORSV-DH and CyCMV-DH based on their complete genomic sequences were constructed and showed closely related to the Japanese isolate ORSV-Cy-1(S83257)and the Japanese isolate CyCMV-Japan(NC_027123),respectively.

Cymbidium hybridumORSVCyCMVcharacterization of genomic sequencemix-infection

金晓香、姚俊汐、尹跃艳、李婷婷、郭淼、钟静、和寿星、陈越、和家卫、鄢波、丁铭

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西南林业大学园林园艺学院,昆明 650224

云南省农业科学院高山经济植物研究所,云南丽江 674100

云南省农业科学院生物技术与种质资源研究所,昆明6502233

大花蕙兰 齿兰环斑病毒 建兰褪绿花叶病毒 基因组序列特征 复合侵染

国家自然科学基金地区科学基金云南省种子种业联合实验室项目云南省乡村振兴科技专项云南省科技重大专项云南省农科院生物技术与种质资源研究所开放项目

32360644202205AR070001-02200202A202302AE0900052023005

2024

10.16420/j.issn.0513-353x.2023-0555

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCD北大核心
影响因子：1.127
ISSN：0513-353X
年,卷(期)：2024.51(8)