Effects of Different Temperatures'Biochar on Soil Fungi Community Structure Under Apple Replantation
Phenolic acids,such as phlorizin,are one of the important factors that cause apple replant disease.In this study,the effects of biochar with different firing temperatures on the fungi community structure and diversity of apple(Malus hupehensis)replanted soil were studied by pot experiments of apple seedlings in replanted soil.There are eight treatments,which are replant soil(control),and addition of 300 ℃ biochar,400 ℃ biochar,500 ℃ biochar,50 mg·kg-1 phlorizin,300 ℃ biochar+phlorizin,400℃ biochar+phlorizin,500 ℃ biochar+phlorizin.After 120 days of pot experiment,the soil fungal communities were determined by high-throughput sequencing technology.The results showed that added biochar could improve the growth of apple seedlings under phlorizin stress,and change the fungal community structure of continuous cropping soil and increase the unique number of soil fungi OUT(operational taxonomic unit).PCoA analysis and UPGMA cluster analysis showed that soil fungal community structure and diversity with the addition of biochar were significantly different from the control soil.The addition of biochar had a certain impact on the structure and function of soil fungi at different species levels,making the abundance of dominant soil fungi change greatly.The addition of biochar had a certain effect on alleviating continuous cropping obstacles by improving the structure of fungal communities.
applereplant diseasephlorizinbiocharhigh-throughput sequencingsoil fungi community
NETL
NSTL
万方数据
