Studies on the Physiological Indexes and Endogenous Hormones Variation During Flower Development in Michelia crassipes
To study the physiological changes and endogenous hormones variation during the flower development of Michelia crassipes,the flower buds from green flower bud stage,purple flower bud stage and the first-open stage were collected,to measure the physiological indexes and the variant endogenous hormones.The results showed that the contents of soluble sugar,soluble protein,chlorophyll and POD activity were decreased during the development of flower bud.The contents of free proline,malondialdehyde,anthocyanin and SOD activity showed the highest value in purple bud stage.According to the UPLC-ESI-MS/MS analysis,a total of 49 hormone substances belonging to 8 classes were detected during the whole developmental process.The flower buds in green flower bud stage accumulated relatively more auxin,salicylic acid(SA)and abscisic acid(ABA),while in purple flower bud stage,the auxin content accounted for the largest proportion of total hormones.The auxin and jasmonic acid(JA)were the predominant hormones in the first-open stage.Additionally,the gibberellin(GA)and ethylene(ETH)contents were increased during the flower development,but the cytokinin(CK)and strigolactones(SLs)contents were decreased.The hormones with significant difference in the content were screened and significantly enriched in the biosynthesis of secondary metabolites pathway.These results indicated that the different physiological activities and hormone interaction patterns worked cooperatively to regulate the flower organs development and anthocyanin accumulation during flower developmental process.Especially,the soluble sugar,soluble protein,auxin,abscisic acid and jasmonic acid might play an important role.
NETL
NSTL
万方数据
