山茶叶片的离体再生及内源激素与不定芽分化的关系
Establishment of Regeneration in Vitro System of Camellia japonica and Relationship Between Endogenous Hormones and Adventitious Bud Differentiation
王建昭 1高园 1刀梅 1张洪烨 1杨自云 1陈龙清 1吴田1
作者信息
- 1. 西南林业大学园林园艺学院,云南省功能性花卉资源及产业化技术工程研究中心,昆明 650224
- 折叠
摘要
以山茶(Camellia japonica)叶片为外植体进行愈伤组织诱导和再分化.结果表明:叶片在MS+5.0 mg·L-1 6-BA+0.1 mg·L-1 2,4-D+5.0 mg·L-1 PVP+3％蔗糖培养基上愈伤组织诱导率最高(79.19％);将愈伤组织转至原配方培养基光照培养(12 h·d-1),可分化出不定芽,分化率为51.32％.不定芽在不定根诱导培养基(1/8MS+0.5 mg·L-1 IBA+1.0 mg·L-1 PVP+2％蔗糖)上生根率最高(50％);生根试管苗移栽至田间,成活率达66.67％.利用液相色谱串联质谱法(LC-MS/MS)检测激素发现,具不定芽分化能力的愈伤组织中,激素信号转导途径、玉米素生物合成代谢途径、α-亚麻酸代谢途径激素含量发生显著变化.在所有检测的内源激素中,有30种(90.9％)含量下调,仅有3种激素含量上调.结果显示,在培养条件一致的前提下,内源激素含量是决定山茶不定芽分化的主要因素.
Abstract
A regeneration system has been established in this study using Camellia japonica leaves as explants.The highest frequency of callus induction(79.19％)was obtained by culturing the leaf explants on the MS media containing 5.0 mg·L-1 6-BA,0.1 mg·L-1 2,4-D,5.0 mg·L-1 PVP and 3％sucrose.Differentiated adventitious buds were generated by directly transferring the explants to the condition of light(12 h·d-1).The frequency of adventitious buds generation was 51.32％.Consequently,adventitious roots were induced from these buds using 1/8MS media containing 0.5 mg·L-1 IBA,1.0 mg·L-1 PVP and 2％sucrose.The highest frequency of root induction was 50％.The C.japonica seedlings rate was 66.67％.The endogenous hormone levels in the tissue-cultured materials were detected using LC-MS/MS.The results demonstrated that the plant hormone signal transduction pathway,zeatin biosynthesis pathway andα-linolenic acid metabolic pathway were changed in the callus with ability to differentiate adventitious buds.Among all the endogenous hormones detected,a total of 30 were down-regulated(90.9％),only three hormones showed up-regulation.The endogenous hormone levels largely determined the induction of callus and differentiation of adventitious buds of C.japonica explants grown under the same culture conditions.
关键词
山茶/再生体系/内源激素/不定芽分化Key words
Camellia japonica/regeneration system/endogenous hormones/differentiation of adventitious bud引用本文复制引用
基金项目
云南省农业基础研究联合专项面上项目(202301BD070001-242)
国家重点研发专项(2019YFD1001005)
云南省教育厅研究生项目(2024Y587)
出版年
2024
NETL
NSTL
万方数据