Establishment of Regeneration in Vitro System of Camellia japonica and Relationship Between Endogenous Hormones and Adventitious Bud Differentiation
A regeneration system has been established in this study using Camellia japonica leaves as explants.The highest frequency of callus induction(79.19％)was obtained by culturing the leaf explants on the MS media containing 5.0 mg·L-1 6-BA,0.1 mg·L-1 2,4-D,5.0 mg·L-1 PVP and 3％sucrose.Differentiated adventitious buds were generated by directly transferring the explants to the condition of light(12 h·d-1).The frequency of adventitious buds generation was 51.32％.Consequently,adventitious roots were induced from these buds using 1/8MS media containing 0.5 mg·L-1 IBA,1.0 mg·L-1 PVP and 2％sucrose.The highest frequency of root induction was 50％.The C.japonica seedlings rate was 66.67％.The endogenous hormone levels in the tissue-cultured materials were detected using LC-MS/MS.The results demonstrated that the plant hormone signal transduction pathway,zeatin biosynthesis pathway andα-linolenic acid metabolic pathway were changed in the callus with ability to differentiate adventitious buds.Among all the endogenous hormones detected,a total of 30 were down-regulated(90.9％),only three hormones showed up-regulation.The endogenous hormone levels largely determined the induction of callus and differentiation of adventitious buds of C.japonica explants grown under the same culture conditions.
Camellia japonicaregeneration systemendogenous hormonesdifferentiation of adventitious bud
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