桃NAC基因家族的鉴定及PpNAC050促进果实果糖积累研究
Identification of Peach NAC Gene Family and Role of PpNAC050 in Promoting Fruit Fructose Accumulation
刘建豪 1荆彦付 2刘月芯 1徐摇光 2于洋 2葛秀秀 3谢华1
作者信息
- 1. 北京农学院生物与资源环境学院,北京 102206;北京市农林科学院生物技术研究所,北京 100097
- 2. 北京市农林科学院生物技术研究所,北京 100097
- 3. 北京农学院生物与资源环境学院,北京 102206
- 折叠
摘要
植物特异性转录因子NAC(NAM,ATAF1/2,CUC2)在调控果实品质中发挥着重要作用.为探索NAC基因在桃(Prunuspersica)果实可溶性糖调控中的作用,在桃基因组中鉴定得到117个NAC基因家族成员(PpNAC),其在桃8条染色体上呈不均匀分布.系统发育关系分析显示,这些PpNAC可归为13个亚类群.克隆了一个在果实发育后期高表达的PpNAC050基因(Prupe.7G014100),其编码蛋白定位于细胞核中,具有转录激活活性.瞬时过表达PpNAC050显著提高了桃果肉中果糖和葡萄糖含量;EMSA和双荧光素酶实验表明,PpNAC050作为转录抑制子,可直接与调控果糖含量的基因——液泡膜单糖外转运蛋白基因PpERDL6-1(PpERDL16)的启动子结合,暗示PpNAC050可通过抑制PpERDL6-1表达来提高果肉中果糖积累.
Abstract
The plant-specific NAC(NAM,ATAF1/2,CUC2)transcription factors play important roles in regulating fruit quality.To explore the role of the NAC in the regulation of soluble sugars in peach(Prunus persica)fruit quality,a total of 117 potential peach NAC members(PpNAC)were identified with an uneven distribution across all eight chromosomes as well as a scaffold.Phylogenetic analyses indicated that these PpNAC were classified into 13 subgroups.The NAC gene PpNAC050 that is highly expressed during later fruit development were further characterized,and it was in nucleus with transcriptional activation.Transient overexpression of PpNAC050 in peach fruits significantly increased fructose content and glucose content in peach fruit flesh.EMSA and dual-luciferase assays showed that PpNAC050,being a transcriptional repressor,directly bound to the promoters of PpERDL6-1(PpERDL16),a vacuolar membrane monosaccharide transporter gene regulating fructose content,suggesting that PpNAC050 positively regulated fructose accumulation by repressing PpERDL6-1 gene expression.
关键词
桃/果糖/NAC/PpNAC050/PpERDL6-1Key words
peach/fructose/NAC gene family/PpNAC050/PpERDL6-1引用本文复制引用
出版年
2024
NETL
NSTL
万方数据