Identification of Peach NAC Gene Family and Role of PpNAC050 in Promoting Fruit Fructose Accumulation
The plant-specific NAC(NAM,ATAF1/2,CUC2)transcription factors play important roles in regulating fruit quality.To explore the role of the NAC in the regulation of soluble sugars in peach(Prunus persica)fruit quality,a total of 117 potential peach NAC members(PpNAC)were identified with an uneven distribution across all eight chromosomes as well as a scaffold.Phylogenetic analyses indicated that these PpNAC were classified into 13 subgroups.The NAC gene PpNAC050 that is highly expressed during later fruit development were further characterized,and it was in nucleus with transcriptional activation.Transient overexpression of PpNAC050 in peach fruits significantly increased fructose content and glucose content in peach fruit flesh.EMSA and dual-luciferase assays showed that PpNAC050,being a transcriptional repressor,directly bound to the promoters of PpERDL6-1(PpERDL16),a vacuolar membrane monosaccharide transporter gene regulating fructose content,suggesting that PpNAC050 positively regulated fructose accumulation by repressing PpERDL6-1 gene expression.
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