Fine-Mapping and KASP Marker Development for the Gene Underlying Green Fruit in Eggplant
An F2 population developing by a white fruit eggplant line E802 and a green fruit eggplant line NY was used for the genetic analysis of the gene underlying green fruit color.The segregation ratio of green fruit and white in the 304 individuals in the F2 population was 3∶1,indicating that the green phenotype of NY is controlled by a single dominant locus.Combing with the BSA-seq and linkage-based QTL mapping,the gene underlying green fruit color of eggplant was fine-mapped into 683.4 kb interval on chromosome 8 using F2 and the F2∶3 population.The genetic distance of this locus is 0.17 cM.Among the 46 genes in the interval,SmAPRR2 was the best candidate gene.The 5.27 kb deletion was detected in the E802,resulting the SmAPRR2 lacking the exon 7 to 11.In addition,a tightly linked to SmAPRR2 KASP marker,22QP296,was development.The accuracies of 22QP296 for predicting the skin color(green or white)in the F2 population and the 194 cultivars were 100％and 94.85％,respectively,indicating that this KASP marker can be used in molecular marker-assisted selection in eggplant breeding.
eggplantfruit colorgene mappingKASP markergreen fruit geneAPRR2
NETL
NSTL
万方数据
