Molecular Markers Assisted Construction of Cmerecta Near-Isogenic Line of Melon Dwarfing Gene
In the early stage,a CmERECTA gene that controls internode length was finely mapped by map cloning,and a marker Cmerecta-dCAPS2 co-separated from melon dwarf was developed.In order to further study its function,the 102 pairs of SSR markers with good polymorphism and relatively uniform distribution on chromosomes were detected by using the melon inbred line TopMark as the reincarnation parent,the dwarf inbred line M406 as the donor parent,and the Cmerecta-dCAPS2 as the foreground selection marker,the background recovery rate of the SSR markers with good polymorphism and relatively uniform distribution on the chromosomes was detected,and the Cmerecta near-isogenic line was constructed under the TopMark genetic background,and its phenotype was statistically analyzed.The results showed that the highest background response rates per plant of BC1F1 and BC2F1 generations were 80.61％and 98.57％,respectively.The phenotypes of the three BC2F2 generation Cmerecta pure lines with the highest background recovery rate showed that the plant height was dwarf and the leaves were rounded.The construction of this near-isogenic line can be used as a basic material for subsequent research on the regulatory mechanism and application of CmERECTA,a dwarf gene in melon.
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