Selection and Validation of Reference Genes for RT-qPCR of Flower Development in Calanthe triplicata
To facilitate studies on gene expression in Calanthe triplicata,nine candidate reference genes were identified and evaluated across three different groups(the whole flower group,the floral structures group and the mixed group)using geNorm,NormFinder,BestKeeper and RefFinder.The results showed although there were slight differences in the ranking of RGs between groups,UBQ1 and UBQ2 were the most stable.To valid the accuracy of the evaluation results,four target genes related to flower development were amplified by RT-qPCR,the results further supported that UBQ1 and UBQ2 were the most optimal for reference genes.
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