Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum
To investigate the regulatory effects of RpACBP3 from Robinia pseudoacacia on the photosynthetic-physiological functions of Nicotiana tabacum,this gene was transferred into N.tabacum'NC89'via Agrobacterium-mediated method,and the relevant indices of RpACBP3 over-expressing tobacco plants were measured using wild-type plants as the control.The results showed that seven strains of N.tabacum with RpACBP3 over-expression were obtained in this experiment.Compared to those in wild-type plants,chlorophyll a,chlorophyll b,and total chlorophyll content considerably increased and carotenoid content decreased in RpACBP3 over-expression strains.Additionally,some indices such as initial fluorescence(Fo),non-photochemical quenching coefficient(qN)decreased,maximum photochemical efficiency(Fv/Fm),actual photochemical quantum efficiency(ΦPSⅡ),photosynthetic rate(Pn),and intercellular CO2 concentration(Ci)increased,whereas others such as stomatal conductance(Gs),transpiration rate(Tr)decreased,stomatal length,and stomatal area increased in RpACBP3-over-expressing-strains.Correlation analysis revealed significant correlations between most of the indicators,and random forest regression showed that RpACBP3 overexpression had the greatest effects on tobacco indices such as chlorophyll a content,ΦPSⅡ,Ci,and stomatal area,respectively.In summary,the RpACBP3 over-expression enhanced the light energy utilization of tobacco plants by increasing the chlorophyll content,improving the efficiency of light absorption and transformation by the photosystem,and promoting the stomatal development.
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