摘要
为了探究刺槐(Robinia pseudoacacia)RpACBP3对光合生理的影响,利用农杆菌介导法将RpACBP3转入到烟草(Nicotiana tabacum)'NC89'中,以野生型为对照,测定RpACBP3过表达烟草的相关指标.结果显示:获得了 7个过表达株系.整体上,RpACBP3过表达株系表现出叶绿素a、叶绿素b、总叶绿素显著增加,类胡萝卜素降低;初始荧光(Fo)和非光化学淬灭系数(qN)下降,最大光化学效率(Fv/Fm)和实际光化学量子效率(ΦPSⅡ)上升;光合速率(Pn)和胞间CO2浓度(Ci)上升,气孔导度(Gs)和蒸腾速率(Tr)下降;气孔长度、气孔面积等明显上升.相关性分析显示,大多数指标之间存在显著相关关系;随机森林回归显示,RpACBP3过表达对烟草叶绿素a、ΦPSⅡ、Ci和气孔面积等指标的影响较大.综上所述,RpACBP3通过提升叶绿素含量、提高光合系统对光能的吸收和转化效率、促进气孔发育等途径来提高烟草植株的光能利用率.
Abstract
To investigate the regulatory effects of RpACBP3 from Robinia pseudoacacia on the photosynthetic-physiological functions of Nicotiana tabacum,this gene was transferred into N.tabacum'NC89'via Agrobacterium-mediated method,and the relevant indices of RpACBP3 over-expressing tobacco plants were measured using wild-type plants as the control.The results showed that seven strains of N.tabacum with RpACBP3 over-expression were obtained in this experiment.Compared to those in wild-type plants,chlorophyll a,chlorophyll b,and total chlorophyll content considerably increased and carotenoid content decreased in RpACBP3 over-expression strains.Additionally,some indices such as initial fluorescence(Fo),non-photochemical quenching coefficient(qN)decreased,maximum photochemical efficiency(Fv/Fm),actual photochemical quantum efficiency(ΦPSⅡ),photosynthetic rate(Pn),and intercellular CO2 concentration(Ci)increased,whereas others such as stomatal conductance(Gs),transpiration rate(Tr)decreased,stomatal length,and stomatal area increased in RpACBP3-over-expressing-strains.Correlation analysis revealed significant correlations between most of the indicators,and random forest regression showed that RpACBP3 overexpression had the greatest effects on tobacco indices such as chlorophyll a content,ΦPSⅡ,Ci,and stomatal area,respectively.In summary,the RpACBP3 over-expression enhanced the light energy utilization of tobacco plants by increasing the chlorophyll content,improving the efficiency of light absorption and transformation by the photosystem,and promoting the stomatal development.
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