茉莉酸甲酯诱导采后脐橙抗青霉病的机制研究
Induction Mechanism of Methyl Jasmonate on Postharvest Navel Orange Fruit Against Blue Mold
陈明 1张洁茹 1杨航云 1王印宝 1郑致远 1曾教科 1陈金印 1付永琦 1向妙莲1
作者信息
- 1. 江西农业大学农学院,江西省果蔬采后处理关键技术与质量安全协同创新中心,果蔬贮藏与保鲜江西省重点实验室,南昌 330045
- 折叠
摘要
以'纽荷尔'脐橙果实为试验材料,采用50 μmol·L-1茉莉酸甲酯(MeJA)熏蒸处理24 h后接种青霉病病原菌意大利青霉(Penicillium italicum)孢子悬浮液,探究MeJA诱导脐橙果实抗青霉病的效应和机理.结果表明,MeJA处理的青霉病病斑直径在发病后1～7 d显著小于对照;过氧化物酶(POD)、多酚氧化酶(PPO)、漆酶(LAC)、苯丙氨酸解氨酶(PAL)、肉桂醇脱氢酶(CAD)、4-香豆酸辅酶A连接酶(4CL)、肉桂酸-4-羟化酶(C4H)活性及相关基因(CsPPO除外)表达量的最高值均显著高于对照;促进了木质素、总酚和类黄酮含量显著增加,而苯丙氨酸和色氨酸含量明显减少.上述结果暗示MeJA处理可能通过调控芳香族氨基酸含量并激发苯丙烷代谢途径相关酶活性和基因表达,促进木质素、总酚和类黄酮含量的积累,诱导脐橙果实增强对青霉病的抗性.
Abstract
In this study,'Newhall'navel orange fruits were used as experimental material and fumigated with 50 μmol·L-1 MeJA for 24 h,then 20 μL 1.25 × 106 spores·mL-1 blue mold pathogen Penicillium italicum spore suspension were injected,which to investigate the induced role of methyl jasmonate(MeJA)treatment on the resistance of postharvest navel orange fruit against blue mold.The results showed the blue mold lesion diameters of MeJA treatment group was significantly smaller than that of the control at 1-7 d after disease onset.The highest activities of POD,PPO,LAC,PAL,CAD,4CL and C4H and their relative gene expression levels except CsPPO in MeJA treatment group were higher than those of the control,respectively.At the same time,MeJA treatment promoted the accumulation of lignin,total phenolics,and flavonoids in navel orange fruits.Moreover,the content of phenylalanine and tryptophan in MeJA treatment group was significantly reduced.The above results indicated MeJA treatment might promote the accumulation of lignin,total phenolics and flavonoids content through regulating aromatic amino acids as well as stimulating the activities and gene expression of phenylpropanoid metabolic pathway-related enzymes,thereby inducing an enhancement in resistance of navel orange fruits against postharvest blue mold.
关键词
脐橙/茉莉酸甲酯/青霉病/诱导抗性/苯丙烷代谢途径Key words
navel orange/methyl jasmonate/blue mold/induced resistance/phenylpropanoid metabolic pathway引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据