Study on the Cold Resistance Function of VaJAZ9 and VaCOI1 Genes in Vitis amurensis
In this study,the VaCOI1 gene from Vitis amurensis Rupr.'Zuoshan-1'were cloned,and the gene function was analysed.The results show that the VaCOI1 gene is located on chromosome 13,and its ORF has a full-length of 1 797 bp,encoding 598 amino acids,and belongs to the F-box family.Clustering analysis indicates that the VaCOIl protein performed a high homology with HuCOI1(Herrania umbratica)and TcCOI1(Theobroma cacao).Bimolecular fluorescence complementation(BiFC)assays indicate that VaJAZ9 can interact with the VaCOI1 protein.The heterologously overexpressing of VaJAZ9 and VaCOI1 in Arabidopsis thaliana shows that at the low temperature,the VaCOI1 overexpressing plants had a higher survival rate and a lower conductivity than the wild type plants,whereas the VaJAZ9 overexpressing plants had a lower survival rate and a higher conductivity than the wild type plants.Quantitative real-time PCR(qRT-PCR)analysis show that the low temperature related genes including AtKIN1,AtCOR15A and AtRD29A were up-regulated in the VaCOI1 overexpressing A.thaliana,whereas these genes were down-regulated in overexpressing VaJAZ9 plants.In conclusion,the results show that the VaCOI1 acts as a positive regulator in response to low temperature stress,whereas the VaJAZ9 acts as a negative regulator.
万方数据
维普
