Whole Genome Resequencing Analysis of'Cuihongli'Plum and Its Early-Ripening Bud Sport Mutation
In order to reveal the molecular basis of formation of'Cuihongli'plum's early-ripening bud sport mutation,this study used high-throughput re-sequencing technology to resequence the whole genomes of'Cuihongli'and its early-ripening bud sport mutation,with an average sequencing depth of 19.40× and 22.82×,with a genome coverage of over 85％.The results showed compared with the reference genome,1 860 745 and 1 931 603 single nucleotide polymorphisms(SNPs),419 134 and 444 892 small fragment insertion/deletion sites(InDels)were detected in'Cuihongli'and its early-ripening bud sport mutation.There are 61 997 and 63 498 nonsynonymous mutations in the coding region,causing a total of 2 391 gene mutations.Metabolic pathway analysis(KEGG)indicated that these genetic variations were mainly involved in the pathways of Ribosome biogenesis in eukaryotes,RNA transport,phenylpropanoid biosynthesis and plant-pathogen interaction.In addition,12 variant genes were annotated with functions in the plant hormone signal transduction pathway.These genes and other related genes variations can affect the activity or specificity of their corresponding proteins or enzymes,leading to changes in the growth and development process,and advancing the mature period of'Cuihongli'plum's early-ripening single plant.
plumbud sport mutationearly-ripeningwhole genome re-sequencing
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