Karyotype Analysis of Four Cultivars of Peppers Based on Fluorescence in situ Hybridization
In order to analyze the karyotype characteristics of different cultivars of peppers,'Zunla-1'(Capsicum annuum L.),'Hainan Huangdenglong'(Capsicum chinense Jacquin),'Muaraparte Rojo'(Capsicum baccatum L.)and'Mijiaolü'(Capsicum frutescens L.),were used in this study.Karyotype analysis was performed using chromosome Fluorescence in situ Hybridization technique(FISH).The results showed that the four peppers were diploid,and the chromosome number was 2n=2x=24.The chromosome length of Zunla-1 is 3.4-7.4 μm,which is mainly metacentric and submetacentric chromosomes,and the karyotype belongs to 20m+3sm+1st type.The chromosome length of Hainan Huangdenglong is 4.6-7.4 μm,which is mainly composed of median centromere and median centromere chromosomes,and the karyotype belongs to 2M+22m.The chromosome length of Muaraparte Rojo was 3.0-4.5 μm,mainly metacentric chromosome,and the karyotype formula was 24m.The length of Mijiaolü chromosome was 3.9-4.9 μm,and it was mainly metacentric chromosome,which was 23m+1sm type.The results of 5S rDNA and 18S rDNA FISH showed that 18S rDNA(20 loci)as probe showed more loci than 5S rDNA(8 loci)on chromosome,individual differences exist among four cultivars of peppers,the number of 5S rDNA loci on chromosome was 2,but the number of 18S rDNA loci was different,Mijiaolü has the highest number(8),followed by Muaraparte Rojo(6),and the lowest is Hainan Huangdenglong(2).
peppercultivarfluorescence in situ hybridizationkaryotype analysis
万方数据
维普
