In order to detect the pathogen of rose plants with yellow and stunt in Huairou,Beijing,small RNAdeep sequencing(sRNA)and reverse transcription-polymerase chain reaction(RT-PCR)were conducted with leaf samples collected from the diseased plants.Broad bean wilt virus 2(BBWV2)was identified after sRNA deep sequencing followed by bioinformatic analyses.To further characterize the identified BBWV2(BBWV2-rose),the complete genome sequence was cloned with primer pairs designed according to the assembled contigs.Sequence analysis showed BBWV2-rose RNA1 and RNA2 were 5 903 and 3 535 nt,respectively.Phylogenetic tree analysis based on RNA1 and RNA2 CP gene sequences suggests that BBWV2-Rose RNA1 and isolate BBWV2-CN:SY:Chenopodium album(MN786954)clustered together,and BBWV2-Rose RNA2 and isolate BBWV2-CN:SY Chenopodium album(MN786955)clustered together,which was closely related.According to Fabavirus classification criteria,the virus should be an isolate of BBWV2.
维普
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