园艺学报2024,Vol.51Issue(10) :2329-2342.DOI:10.16420/j.issn.0513-353x.2023-0923

不同倍性菊花远缘杂交亲和性及子代倍性研究

Studies on Distant Crossing Compatibility and Hybrid Ploidy in Different Ploidy Chrysanthemums

叶雨晴 孙丽萍 张一驰 李毅 徐俊 马琛悦 牛雅静 张蒙蒙 戴思兰 黄河
园艺学报2024,Vol.51Issue(10) :2329-2342.DOI:10.16420/j.issn.0513-353x.2023-0923

不同倍性菊花远缘杂交亲和性及子代倍性研究

Studies on Distant Crossing Compatibility and Hybrid Ploidy in Different Ploidy Chrysanthemums

叶雨晴 1孙丽萍 2张一驰 1李毅 1徐俊 1马琛悦 1牛雅静 3张蒙蒙 3戴思兰 1黄河1
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作者信息

  • 1. 北京林业大学园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,园林环境教育部工程研究中心,林木花卉遗传育种教育部重点实验室,北京 100083
  • 2. 北京市园林绿化科学研究院,绿化植物育种北京市重点实验室,北京 100102
  • 3. 北京市植物园,北京市花卉园艺工程技术研究中心,北京 100093
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摘要

以菊属8个野生种及变种、10个栽培种为亲本,设计了 60个不同倍性菊花间的远缘杂交组合,采用常规压片法对所有杂交亲本及部分子代的倍性进行鉴定,以分析不同倍性菊花杂交的亲和性.结果表明,不同倍性的菊花栽培种与野生种间远缘杂交亲和性存在显著差异,四倍体×四倍体、四倍体×五倍体亲和指数最高,五倍体×二倍体、六倍体×二倍体亲和指数最低,但所有倍性差异的杂交组合均能结实、出苗.远缘杂交子代倍性变化十分明显,同倍性组合的子代倍性几乎与亲本一致,不同倍性的杂交组合子代倍性存在多样性.此外,通过使用低倍性菊属野生种间或低倍性野生种与栽培菊花杂交获得的中间种质作为桥梁亲本,能够在一定程度上克服远缘杂交不亲和或亲和性差的问题.

Abstract

The 60 distant crossing combinations were designed by utilizing ten cultivars,eight wild species and variants of Chrysanthemum genus as the parents,and identified the ploidy of all parents and certain hybrids using the conventional tableting method,aiming to assess the crossing compatibility.The results indicated notable variations in compatibility of distant crossing among various ploidy cultivars and wild species in Chrysanthemums.The combinations of tetraploid × tetraploid and tetraploid × pentaploid exhibited the highest compatibility indexes,while pentaploid × diploid and hexaploidy × diploid had the lowest compatibility indexes.It was worth noting that all crossing combinations with differences in ploidy were able to set seeds and germinate.The ploidy variation of the hybrids was readily obvious,the hybrids resulting from crosses with the same ploidy exhibited similar ploidy to their respective parents,but there was diversity observed in the ploidy of hybrids resulting from different ploidy crossing combinations.In addition,the problems of distant crossing,which involve limited or inadequate compatibility,can be partially overcome by utilizing intermediate germplasms derived from crossing low-ploidy wild species or low-ploidy wild species with cultivars of chrysanthemum as bridging parents.

关键词

菊花/野生种/远缘杂交/杂交亲和性/倍性/桥梁亲本

Key words

Chrysanthemum/wild species/distant crossing/crossing compatibility/ploidy/bridging parent

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基金项目

中央高校基本科研业务费专项资金项目(QNTD202306)

北京市植物园管理处科技课题(BZ202203)

出版年

2024
园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCD北大核心
影响因子:1.127
ISSN:0513-353X
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