Studies on Distant Crossing Compatibility and Hybrid Ploidy in Different Ploidy Chrysanthemums
The 60 distant crossing combinations were designed by utilizing ten cultivars,eight wild species and variants of Chrysanthemum genus as the parents,and identified the ploidy of all parents and certain hybrids using the conventional tableting method,aiming to assess the crossing compatibility.The results indicated notable variations in compatibility of distant crossing among various ploidy cultivars and wild species in Chrysanthemums.The combinations of tetraploid × tetraploid and tetraploid × pentaploid exhibited the highest compatibility indexes,while pentaploid × diploid and hexaploidy × diploid had the lowest compatibility indexes.It was worth noting that all crossing combinations with differences in ploidy were able to set seeds and germinate.The ploidy variation of the hybrids was readily obvious,the hybrids resulting from crosses with the same ploidy exhibited similar ploidy to their respective parents,but there was diversity observed in the ploidy of hybrids resulting from different ploidy crossing combinations.In addition,the problems of distant crossing,which involve limited or inadequate compatibility,can be partially overcome by utilizing intermediate germplasms derived from crossing low-ploidy wild species or low-ploidy wild species with cultivars of chrysanthemum as bridging parents.