Identification and Bioinformatics Analysis of the HSF Family Gene in Pinellia ternata
In this study,the PtHSF genes involved in the high-temperature-induced ST of Pinellia ternata were identified and characterized.Twenty-two members of the PtHSF gene family were accordingly identified in P.ternata,which were unevenly distributed across its 10 chromosomes.All PtHSF genes were found to contain HSF-characteristic domains(DBD conserved domains),and phylogenetic tree analysis revealed that these genes can be divided into three major subclasses.PtHSF genes in the same subclass were established to have similar gene structures and conserved motifs,and encode similar amino acids.Subcellular localization prediction indicated that 81.82％of the PtHSF protein is located in the nucleus,with the remainder being distributed outside the cell.Collinearity analysis revealed that the PtHSF gene family has only a single collinear pair,and that PtHSF and rice OsHSF have a close homologous evolutionary relationship.Expression pattern analysis revealed that the expression of PtHSF family members differs significantly among different plant tissues,with most members being observed to be highly expressed in roots and tubers.In response to exposure to high temperature stress,the induced expression of 12 PtHSF genes and inhibited expression of eight PtHSF genes were detected.
万方数据
维普
