Effects of Irrigation Before Soil Freezing on Bacterial Community Composition and Available Nutrient Content in'Fuji'Apple Rhizosphere
In this study,the 5-year-old'Fuji'apple trees were used as materials.After full irrigation before soil freezing,samples were taken in the next January and April to analyze the changes of bacterial community composition and available nutrients in rhizosphere soil.The results showed that irrigation before freezing not only increased soil moisture status and stabilized soil temperature,but also significantly increased soil available nutrient content,and reduced soil organic matter content.The content of soil available nitrogen,phosphorus and potassium increased by 15.83％,21.13％and 8.22％,while the content of organic matter decreased by 19.86％.In addition,the root density,root activity and root nitrogen content increased by 10.3％,15.6％and 13.3％,respectively in early spring.Quantitative PCR analysis of functional genes showed that the irrigation before soil freezing significantly increased the number of soil bacteria and fungi,including ammonia-oxidizing archaea(AOA),nitrogen fixing bacteria and denitrification bacteria abundance increased 35.18％,18.12％and 19.53％,respectively,thus promoting soil nitrification and denitrification.
appleirrigation before soil freezingrhizosphere bacterial communitysoil available nutrientssoil nitrogen conversion
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