Effects of Coronatine on Endogenous Hormone Content and Activities of Key Anthocyanin Synthesis Enzymes in'Flame Seedless'Grape Berry
'Flame Seedless'grape,the main red grape cultivar in Xinjiang,was used as the test material in this study.To explore the mechanism of action of the plant growth regulator coronatine(COR)in promoting anthocyanin accumulation in grape peel and improving fruit coloring,it determined the effects of COR on fruit quality,anthocyanin,endogenous hormones,and the activity of key enzymes involved in anthocyanin synthesis.Three COR concentrations were prepared for solution dipping treatments in the early fruit color conversion stage(47 days after full blooming).The results showed that the treatment with all three concentrations of COR could improve fruit quality,significantly increase anthocyanin content in peel,and increase indoleacetic acid(IAA),gibberellin(GA),jasmonic acid(JA)content in some periods as well as the abscisic acid(ABA)content at the late stage of color conversion.The activities of key enzymes involved in anthocyanin synthesis,namely PAL,CHS,CHI,F3H,DFR,ANS,and UFGT,were increased,which promoted the synthesis and accumulation of anthocyanins and improved fruit coloring.Among the three treatments,the treatment with COR diluted 3 000 times yielded the excellent results.Compared with the control(water),the treatment with COR diluted 3 000 times exhibited 12.34％higher total soluble sugars content,22.40％lower total organic acids content,44.70％higher TSS/TA ratio,56.88％higher anthocyanin content,and 80.04％higher JA content.Correlation analysis showed that the JA content was positively correlated with the activity of key enzymes involved in anthocyanin synthesis in most periods,and both JA content and key anthocyanin synthesis enzyme activities were positively correlated with the anthocyanin content.Comprehensive analysis showed that COR treatment in the early stage of fruit coloration mainly promoted the synthesis of JA in berry,thereby increasing the activity of key enzymes involved in the anthocyanin synthesis pathway,which promoted anthocyanin synthesis in the peel and enhanced the appearance fruit color.
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