Preliminary Study on the Prevention and Control Effect of Bacillus amyloliquefaciens Strain 5 on Cucumber Green Mottled Mosaic Virus Disease on Watermelon
In order to screen for effective microbial agents against cucumber green mottled mosaic virus(CGMMV)disease on watermelon,Paenibacillus polymyxa 246-1,Bacillus velezensis 4-2,and B.amyloliquefaciens 5 microbial agents were applied in advance on watermelon seedlings inoculated with CGMMV.The results showed that P.polymyxa 246-1,B.velezensis 4-2,and B.amyloliquefaciens 5 control effects of CGMMV disease reached 59.89％,46.71％,and 65.77％,respectively.Among them,B.amyloliquefaciens 5 had the best prevention and control effect.Further determination of physiological and biochemical indicators and phenotypic changes in watermelon seedlings on the 21st day was conducted to verify the prevention and control of CGMMV disease by pre-treatment with B.amyloliquefaciens.The results showed that the application of B.amyloliquefaciens 5 microbial agent in advance significantly increased the plant height,root length,fresh weight,and dry weight of watermelon seedlings by 5.93 cm,2.92 cm,2.48 g,and 2.09 g compared to the control group;the net photosynthetic rate,stomatal conductance,and transpiration rate of watermelon seedlings significantly increased by 351.00％,133.33％,and 91.30％,while the intercellular CO2 concentration significantly decreased by 25.88％;simultaneously,the activities of SOD,POD,PAL,and CAT in watermelon seedlings were significantly increased by 100.37％,126.46％,98.45％,and 98.99％.In conclusion,B.amyloliquefaciens 5 can improve the resistance of watermelon to CGMMV by improving the growth and development of plants,the photosynthesis ability of leaves and the activities of resistant enzymes.
watermeloncucumber green mottle mosaic virusBacillus amyloliquefaciensbiological prevention and control
万方数据
维普
