Establishment of Agrobacterium rhizogenes-Mediated Genetic Transformation System in Vegetable Pea
This study established a simple and efficient Agrobacterium rhizogenes-mediated genetic transformation system in vegetable pea.The vegetable pea seeds of'Zhewanl'were sown into pots with wet sterile vermiculite.The hypocotyls of vegetable pea seedlings were cut at the junction of cotyledon node and hypocotyl and the residual seedlings were selected as explants.The hairy roots were induced with Agrobacterium rhizogenes AR1 193 strain harboring the construct of pCAMBIA3301-GUS.The results indicated that 100％of the inoculated explants could produce hair roots.The effect of different seedling age of explant on hairy root induction was also investigated.It was found that the 7-day-old seedling was favorable for hair root induction and the number of hairy root per seedling was 14.60.Further,the hair roots were amplified by RT-PCR of GUS gene and stained with the GUS dye.It was proven that the exogenous GUS gene could be successfully expressed in the hair roots and 35.21％of the hair roots were transgenic positive roots.
万方数据
维普
