Identification and Expression Analysis of Trihelix Gene Family in Common Bean
In this study,bioinformatics analysis of the common bean Trihelix gene family were performed,and the expression of the PvTrihelixes under adversity stress were examined using transcriptomic data(RNA-seq)and fluorescence quantitative PCR(qRT-PCR).The results showed that 40 PvTrihelixes genes were identified in common bean genome,which unevenly distributed on 11 chromosomes.Phylogenetic analyses classified PvTrihelixes into five subfamilies(GT-1,GT-2,GTγ,SIP1 and SH4).Five subfamilies were essentially similar in terms of gene structure and conserved motifs.Covariance analysis revealed there are 5 pairs of tandem repeat and 9 pairs of fragment repeat in PvTrihelixes.The promoter analysis revealed that PvTrihelixes contain ciss-acting elements related to growth and development,light,hormones,and stress response.Tissue expression analysis revealed the presence of tissue expression specificity in PvTrihelix,and the genes with higher expression levels in flowers all belonged to members of the GT-2 subfamily and the SIP1 subfamily.The qRT-PCR results showed that PvTrihelix6/7/10/11 were significantly up-regulated under drought(6％PEG),PvTrihelix1/21/25/26/31/35 were up-regulated in response to low temperature(4 ℃),and PvTrihelix21/23/25/27/28/40were significantly up-regulated under salt(100 mmol·L-1 NaCl)stress.
common beanTrihelix gene familyevolutionary analysisexpression patternabiotic stress
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