Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA
To clarify the regulation function of vegetable soybean GmDi19-3 promoter,the expression patterns of GmDi19-3 gene in different tissues and in response to salt stress and exogenous plant growth regulators were analyzed.The promoter of GmDi19-3 was cloned and cis-acting element composition was analyzed.The promoter of GmDi19-3 was fused with the GUS gene in the expression vector which was stably expressed in Arabidopsis.The promoter activity of GmDi19-3 was detected in the transgenic plants.Typical salt stress and exogenous ABA and MeJA signal inducible expression patterns of GmDi19-3 were observed.The promoter of GmDi19-3 harbored abiotic stress and hormone signal responsive elements.Based on the GUS staining and activity detection assays,it was verified that salt stress and exogenous ABA and MeJA signals obviously enhanced the promoter activity of GmDi19-3 in the leaf,stem and root of transgenic plants.These results suggested that GmDi19-3 promoter played key roles in response to salt stress,and exogenous ABA and MeJA signals.
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