菜用大豆GmDi19-3启动子对盐胁迫和外源ABA、MeJA的响应
Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA
冯志娟 1刘娜 1张古文 1卜远鹏 1王斌 1龚亚明1
作者信息
- 1. 农业农村部南方豆类蔬菜种质创新与分子育种重点实验室(部省共建),省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江省农业科学院蔬菜研究所,杭州 310021
- 折叠
摘要
为阐明菜用大豆GmDi19-3启动子的调控功能,分析了 GmDi19-3的盐胁迫诱导性、外源植物生长调节剂信号诱导性和组织表达特异性,克隆获得GmDi19-3启动子序列并分析其顺式作用元件组成,构建GmDi19-3启动子融合GUS报告基因表达载体并在拟南芥中稳定表达,检测启动子在转基因植株中的活性.GmDi19-3呈现典型的盐胁迫和外源ABA、MeJA信号诱导表达特性,GmDi19-3启动子区域包含逆境胁迫及激素诱导表达元件.GUS组织染色和活性检测显示,GmDi19-3启动子具有活性,在叶片、茎和根中明显表达,盐胁迫及外源ABA和MeJA信号能显著诱导GmDi19-3启动子活性增强.这些研究结果表明GmDi19-3启动子在菜用大豆盐胁迫和外源ABA、MeJA信号应答过程中发挥重要作用.
Abstract
To clarify the regulation function of vegetable soybean GmDi19-3 promoter,the expression patterns of GmDi19-3 gene in different tissues and in response to salt stress and exogenous plant growth regulators were analyzed.The promoter of GmDi19-3 was cloned and cis-acting element composition was analyzed.The promoter of GmDi19-3 was fused with the GUS gene in the expression vector which was stably expressed in Arabidopsis.The promoter activity of GmDi19-3 was detected in the transgenic plants.Typical salt stress and exogenous ABA and MeJA signal inducible expression patterns of GmDi19-3 were observed.The promoter of GmDi19-3 harbored abiotic stress and hormone signal responsive elements.Based on the GUS staining and activity detection assays,it was verified that salt stress and exogenous ABA and MeJA signals obviously enhanced the promoter activity of GmDi19-3 in the leaf,stem and root of transgenic plants.These results suggested that GmDi19-3 promoter played key roles in response to salt stress,and exogenous ABA and MeJA signals.
关键词
菜用大豆/Di19-3/启动子/GUS活性/盐胁迫/ABA/MeJAKey words
vegetable soybean/Di19-3/promoter/GUS activity/salt stress/ABA/MeJA引用本文复制引用
出版年
2024
NETL
NSTL
万方数据