Transcriptome Analysis of the Response of Tree Peony'Hu Hong'Under High Temperature Stress
To analyze the molecular regulation mechanism of tree peony(Paeonia suffruticosa)in southern China,'Hu Hong'was selected as the test material,the leaves treated at 20 ℃ and 40 ℃ were collected after 0,2,6,12 and 24 h,respectively.Transcriptomics and physiological and biochemical indicators were used to explore the differences in gene expression at different treatment times under high temperature stress.The result showed that the leaves showed water loss and biofilm damage,the relative conductivity of leaves and the proline content increased significantly with the prolongation of high temperature stress time.Free proline,relative conductivity and soluble protein were identified as important indicators to measure the changes of physiological mechanism in the response of peony to high temperature stress.A total of 492 693 unigene and 840 509 transcripts were generated by RNA-seq.36 174 differentially expressed genes(DEGs)were annotated in 5 databases including GO and KEGG.The GO analysis showed that the main enrichment pathways were the reaction pathway to heat and the cycle pathway of reduced pentose phosphate.KEGG enrichment analysis showed that DEG is involved in signal transduction,photosynthesis and metabolism play a major role in responding to high temperature stress.Among the DEGs,multiple candidate genes and transcription factors involved in heat stress have been screened out,such as heat shock transcription factors PSHSFA2b,PSHSFA3,PSHSFB2a,PSHSFB2b,PSHSFB4,PSHSFC1 and some NAC,MYB,bHLH family genes.The expression of 11 selected genes under high temperature stress was detected by qRT-PCR,and the results were similar to RNA seq.
peonyhigh temperature stresstranscriptomephysiological and biochemical indicators
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