Cloning and ABA Transport Function Analysis of CsNPF6.1/6.3 in Tea Plants
To explore the hormone transport characteristics of tea(Camellia sinensis)NPF transporter,two complete NPF genes(CsNPF6.1 and CsNPF6.3)were cloned from'Longjing 43'leaves.The CDS(coding sequence)of CsNPF6.1 and CsNPF6.3 were 1 665 bp and 1 788 bp encoding 554 and 595 amino acids,respectively.Phylogenetic analysis showed that CsNPF6.1 and CsNPF6.3 had the highest identical sequence identity with ClNPF(Camellia lanceoleosa),which were 98.19％and 97.82％respectively.Both CsNPF6.1 and CsNPF6.3 were predicted to be located in the plasma membrane and were typical membrane transporters.Tissue-specific expressions revealed that they were expressed in tender leaves,mature leaves,and roots,and mainly accumulated in tender leaves.Under ABA treatment,the expression levels of CsNPF6.1 and CsNPF6.3 were significantly decreased in tender leaves,but significantly increased in roots.According to the identification of ABA-dependent yeast two-hybrid system,CsNPF6.1 and CsNPF6.3 could promote the growth of transgenic yeast cells and could significantly increase the content of ABA in yeast cells under 100 μmol·L-1 ABA condition.In conclusion,both CsNPF6.1 and CsNPF6.3 in tea plants have the function to transport ABA.
tea plantCsNPF6.1CsNPF6.3gene cloningABA transport
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