Study on the Regulation of Lactate Dehydrogenase on Mycelia Growth of Pleurotus ostreatus Under Low Temperature and Light
In order to study the effect of lactate dehydrogenase on mycelial growth of Pleurotus ostreatus under the environmental stimulation of low temperature and light,the expression profiles of lactate dehydrogenase were analyzed,and the influences of exogenous lactate dehydrogenase inhibitor,lactic acid,and pyruvic acid on the mycelial growth were observed.The results showed that lactate dehydrogenase could accelerate mycelial growth in 12 h of light alternated with 12 h of darkness at 15 ℃.Inhibition of lactate dehydrogenase enzyme activity would cause the activity of pyruvate dehydrogenase(PDH)to be decreased by 15.02％,which giving rise to the decreasing flux of pyruvic acid into the tricarboxylic acid cycle,the production of ATP reduced by 14.78％,and the intracellular contents of lactic acid and pyruvic acid increased by 18.97％and 12.57％,respectively.The accumulation of lactic acid and pyruvic acid could induce increased activities of alcohol dehydrogenase,which leading to the increase of NAD+content and the imbalance of NADH/NAD+ratio,and therefore the inhibition of mycelial growth.
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