Digital PCR(dPCR)is a newly emerged technology to quantify the absolute nucleic acid content with high-sensitivity in recent years.It can achieve absolute quantification of the initial concentration of the sample without the need to rely on reference or standard curve,with characteristics of high sensitivity,specificity,and accuracy.This article discusses the basic principle of dPCR,and reviews its applications in the detection of genetically modified plants,plant pathogen diagnosis,copy number variation,gene expression research,and further perspectives for the application of dPCR in plants.
digital PCRplant researchgenetically modified plantsplant pathogen diagnosisabsolute quantification
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