The Quantitative Real-time PCR on the Detection and Assessment of Acinetobacter Baumanii Drug Resistance Genes in The Sputum Samples of ICU Patients
Objective To study the effect of the quantitative real-time polymerase chain reaction(PCR)on the detection and assessment of acinetobacter baumanii drug resistance genes in the sputum samples of intensive care unit(ICU)patients.Methods A total of 68 ICU patients enrolled in Nanyang Central Hospital from March 2021 to December 2022 were selected for the trial,and sputum samples were collected respectively.The quantitative real-time PCR was applied to detect the acinetobacter baumanii drug resistance genes.The detection rates of acinetobacter baumanii and carbapenem-resistant acinetobacter baumanii were counted;the drug sensitive test results of carbapenem-resistant acinetobacter baumanii were observed.OXA-15 genes and OXA-23 drug-resistant genes were analyzed.Results In sputum samples of 68 ICU patients,33 strains of Acinetobacter baumannii were detected by traditional culture,and the positive detection rate was 48.53%.A total of 23 carbapenem-resistant Acinetobacter baumannii were detected,and the positive detection rate was 33.82%.OXA-51 gene test showed 37 strains of Acinetobacter baumannii(54.41%).OXA-23 positive results showed that there were 25 carbapenem-resistant Acinetobacter baumannii,and the positive detection rate was 36.76%.The proportion of Acinetobacter baumannii resistant to carbapenems accounted for 75.76%.Carbapenem-resistant Acinetobacter baumannii was resistant to most drugs,and the drugs with low resistance were cefoperazone sulbactam,minocycline,tigacycline and colistin.There were no significant difference about the positive bacterial detection rates based on the conventional cultivation and OXA-51 drug-resistance genes(P＞0.05);Kappa values were 0.879,demonstrating the good consistency based on two examination modes.There were no significant difference about the positive bacterial detection rates based on the conventional cultivation and OXA-23 drug-resistance genes(P＞0.05);Kappa values were 0.712,demonstrating the general consistency based on two examination modes.Conclusion The quantitative real-time PCR method has obvious effect,and can be the main method to detect the drug resistance gene of Acinetobacter baumannii.
acinetobacter baumaniiquantitative real-timepolymerase chain reactionintensive care unit
国家科技期刊平台
NSTL
万方数据
维普
