Objective To analyze the clinical application value of pre-treatment of microbial culture system in matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)for rapid identification of pathogenic bacteria in blood culture positive samples using microbial culture equipment(HB&L).Methods A total of 120 positive samples of single bacterial infection identified by routine laboratory methods from January 2022 to December 2022 were collected.All samples were subjected to pre-treatment using the HB&L microbial culture system and separation gel coagulation tube method.The identification results of traditional methods were used as the"gold standard"control,and MALDI-TOF MS was used for identification.The identification rates of MALDI-TOF MS rapid identification under pre-treatment of HB&L microbial culture system and separation gel assisted coagulation tube method were compared;In addition,the rapid identification results of bacterial genera in MALDI-TOF MS samples under different scores were compared between the pre-treatment of HB&L microbial culture system and the separation gel coagulation tube method.Results Under the pre-treatment of HB&L microbial culture system,the number of MALDI-TOF MS rapid identification cases was 116,with an identification rate of 96.67%;The number of rapid identification cases of MALDI-TOF MS under the pre-treatment of separation gel coagulation promoting tube method was 92,with an identification rate of 76.67%.The difference in identification rates between the two pre-treatment methods was statistically significant(P＜0.05);The rapid identification rate of bacterial genera in the MALDI-TOF MS samples of the HB&L microbial culture system was higher than that of the separation gel coagulation tube method at a score of 3.000～2.300,and the difference was statistically significant(P＜0.05);There was no statistically significant difference in the bacterial identification rates between the two pre-treatment methods at scores of 2.299～2.000,1.999～1.700,and＜1.700(P＞0.05).Conclusion The HB&L microbial culture system is a high-quality pre-treatment method for rapid identification of pathogenic bacteria in blood culture positive samples by MALDI-TOF MS,which can significantly improve the identification rate and is worthy of application.
pretreatment of HB&L microbial culture system/MALDI-TOF MS rapid identification/positive sample pathogen/bloodstream infection/pretreatment of separation gel coagulation promoting tube method
维普
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