微生物培养体系预处理在MALDI-TOF MS快速鉴定血培养阳性病原菌的应用价值

The Application Value of microbial Culture System Pretreatment in MALDI-TOF MS Rapid Identification of Blood Culture positive Pathogenic Bacteria

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中文摘要：目的分析微生物培养仪(HB&L)微生物培养体系预处理在基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF MS)快速鉴定血培养阳性样本病原菌的临床应用价值.方法收集 2022 年 1 月至 2022 年 12 月安阳市人民医院实验室常规方法鉴定的 120 份血培养报警后单一细菌感染的阳性样本,所有样本均接受HB&L微生物培养体系预处理、分离胶促凝管法预处理,将传统方法的鉴定结果作为"金标准"对照,运用MALDI-TOF MS鉴定.对比HB&L微生物培养体系预处理、分离胶促凝管法预处理下MALDI-TOF MS快速鉴定的鉴定率;另外对比HB&L微生物培养体系预处理、分离胶促凝管法在不同分值下MALDI-TOF MS样本细菌菌属快速鉴定结果.结果 HB&L微生物培养体系预处理下MALDI-TOF MS快速鉴定例数为 116 例,鉴定率为 96.67%;分离胶促凝管法预处理下MALDI-TOF MS快速鉴定例数为92例,鉴定率为76.67%,两种预处理方法鉴定率对比差异有统计学意义(P＜0.05);在3.000～2.300分值下HB&L微生物培养体系MALDI-TOF MS样本细菌菌属快速鉴定率均高于分离胶促凝管法,差异有统计学意义(P＜0.05);在2.299～2.000、1.999～1.700、＜1.700分值下两种预处理方法菌属鉴定率对比差异无统计学意义(P＞0.05).结论 HB&L微生物培养体系是MALDI-TOF MS快速鉴定血培养阳性样本病原菌优质的预处理方法,可大幅提升鉴定率,值得应用.

外文摘要：Objective To analyze the clinical application value of pre-treatment of microbial culture system in matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)for rapid identification of pathogenic bacteria in blood culture positive samples using microbial culture equipment(HB&L).Methods A total of 120 positive samples of single bacterial infection identified by routine laboratory methods from January 2022 to December 2022 were collected.All samples were subjected to pre-treatment using the HB&L microbial culture system and separation gel coagulation tube method.The identification results of traditional methods were used as the"gold standard"control,and MALDI-TOF MS was used for identification.The identification rates of MALDI-TOF MS rapid identification under pre-treatment of HB&L microbial culture system and separation gel assisted coagulation tube method were compared;In addition,the rapid identification results of bacterial genera in MALDI-TOF MS samples under different scores were compared between the pre-treatment of HB&L microbial culture system and the separation gel coagulation tube method.Results Under the pre-treatment of HB&L microbial culture system,the number of MALDI-TOF MS rapid identification cases was 116,with an identification rate of 96.67%;The number of rapid identification cases of MALDI-TOF MS under the pre-treatment of separation gel coagulation promoting tube method was 92,with an identification rate of 76.67%.The difference in identification rates between the two pre-treatment methods was statistically significant(P＜0.05);The rapid identification rate of bacterial genera in the MALDI-TOF MS samples of the HB&L microbial culture system was higher than that of the separation gel coagulation tube method at a score of 3.000～2.300,and the difference was statistically significant(P＜0.05);There was no statistically significant difference in the bacterial identification rates between the two pre-treatment methods at scores of 2.299～2.000,1.999～1.700,and＜1.700(P＞0.05).Conclusion The HB&L microbial culture system is a high-quality pre-treatment method for rapid identification of pathogenic bacteria in blood culture positive samples by MALDI-TOF MS,which can significantly improve the identification rate and is worthy of application.

外文关键词：

pretreatment of HB&L microbial culture systemMALDI-TOF MS rapid identificationpositive sample pathogenbloodstream infectionpretreatment of separation gel coagulation promoting tube method

作者：

杨献飞、李晓燕

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作者单位：

安阳市人民医院检验科,河南安阳 455000

北京市房山区良乡医院检验科,北京 100000

关键词：

HB&L微生物培养体系预处理 MALDI-TOF MS快速鉴定阳性样本病原菌血流感染分离胶促凝管法预处理

出版年：

2024

DOI：

10.12385/j.issn.2096-1278(2024)04-0012-04