Study on the Influence of Procoagulant and Anticoagulant on the Detection Results of HBsAg in Enzyme-Linked Immunosorbent assay
Objective To analyze the effect of procoagulation and anticoagulation samples on the detection of hepatitis B virus surface antigen(HBsAg)in enzyme-linked immunosorbent assay(ELISA).Methods From January 2022 to June 2022,3680 unpaid blood donors in our central blood station were investigated.Two specimens were collected for each donor.One specimen was stored after the procoagulation treatment and one specimen after the anticoagulant treatment.The same testing instruments and reagents were used for all blood samples,and ELISA was used to detect HBsAg under the same conditions;and add anticoagulants according to the proportion of HBsAg weakly positive reference materials for testing.Comparison of sample absorbance to standard absorbance ratio(S/CO value)for ELISA detection of HBsAg between procoagulant and anticoagulant samples.Results ELISA was used to detect HBsAg in 48 cases of procoagulant samples,with a positive rate of 1.30%;30 anticoagulant samples were tested for HBsAg using ELISA,with a positive rate of 0.82%.The positive rate of using procoagulant samples for ELISA detection of HBsAg is higher than that of anticoagulant samples,and the difference is statistically significant(P＜0.05).There were inconsistencies in the HBsAg test results of 18 out of 3680 voluntary blood donors,including both procoagulant and anticoagulant samples.The S/CO values of HBsAg test in procoagulant samples were generally higher than those in anticoagulant samples.After repeated experiments,the retest results of the 18 samples mentioned above were consistent and all were positive.The results of the repeated experiments were the same as those of the procoagulant samples.After adding anticoagulants to HBsAg quality control serum in proportion,the amount of anticoagulants continuously increases,and the HBsAg content and S/CO value gradually decrease.The S/CO values of coagulation promoting samples used for ELISA detection of HBsAg were higher than those of anticoagulant samples,and the difference was statistically significant(P＜0.05).Conclusion During the enzyme-linked immunosorbent assay,the missed diagnosis conditions of HBsAg could be found in the anticoagulant specimens.Thus the procoagulant specimen shall be kept to ensure the examination accuracy.
procoagulant specimensanticoagulant specimensenzyme-linked immunosorbent assayhepatitis B surface antigen
国家科技期刊平台
NSTL
万方数据
维普
