Cloning and Expression Analysis of Sucrose-phosphate Synthase Ⅱ Gene from Sugarcane
To achieve the ultimate aim of increasing sucrose content in the sugarcane stem, it is necessary to investigate the principal rate-limiting steps in the pathway of sucrose synthesis. It has been suggested that sucrose phosphate synthase (SPS) is a key rate-limiting enzyme in sucrose formation and it also influences sucrose accumulation and carbon partitioning processes. Relative expression of SPSⅡ gene occupies 40% of the total transcription of SPS gene, which shows that the cloning and expression of SPSⅡ gene influences sucrose accumulation process. This research focused on molecular cloning and expression analysis of SFSⅡ gene in sugarcane. The SPSⅡ cDNA fragment was isolated from sugarcane, which contained a 3 183 bp open reading frame (ORF), encoding 1 060 amino acids. Its Genbank accession no. EU 269038. Relative expression of SPSⅡ gene was determined in the different tissues of sugarcane at different time of sugar accumulation by real-time PCR. The results showed highest relative expression of SPSⅡ gene in the canes at early stage during accumulation process of sucrose, but highest in the leaves at middle stage. In the same photosynthetic tissue, relative expression of SPSⅡ was higher at early stage during accumulation process of sucrose than those at middle stage and later stage. Fig 6, Ref 11
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万方数据
维普
