Detecting Genetic Diversity of Dendrobium Tissue Culture Seedlings with ISSR Method
In order to investigate the genetic diversity of the tissue culture seedlings of Dendrobium species, molecular marker ISSR was used to detect the interspecific genetic differences among D.officinale, D.denneanum and D.nobile.Genetic diversities of different capsules of the same plant, different plants of the same capsule and different subcultures of the same plant were analyzed.Results showed that: (1) the genetic variance among D.officinale, D.denneanum and D.nobile was at a higher level, with the polymorphism percentage and the similarity coefficient 80.59％ and 0.32 respectively.The genetic distance between D.officinale and D.nobile was the smallest (0.406), and the distance between D.denneanum and D.nobile the largest (1.099).(2) For the different capsules of the same plant, POPGENE analysis showed that the largest genetic diversity existed within D.nobile (77.78％), followed by D.officinale (58.82％) and D.denneanum (28.57％).One possible reason was the larger number of capsules ofD.nobile than ofD.denneanum.The genetic distances were 0.338, 0.301 and 0.191, respectively.(3) Genetic diversity also existed between different plants of the same capsule.The polymorphism percentage of D.nobile was the largest (25.00％),followed by D.denneanum (11.11％) and D.officinale (7.14％).The genetic distances were 0.207, 0.047 and 0.030, respectively.(4) The electrophoresis strips of the 4 subcultures were consistent, with no variation in the process of cultivation.The results,therefore, suggested that ISSR method is suitable for studying the genetic diversity of Dendrobium species.Fig 4, Tab 7, Ref 12
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万方数据
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