Responses of two maize inbred lines of different aluminium tolerance to Al-induced oxidative stress
Aluminium (A1) toxicity seriously inhibits root growth and development of plants in acid soils.Root growth inhibition is the most obvious phenotype observable shortly after Al exposure.Previous reports believed Al triggered oxidative stress and further activated defense responses of plant.In China maize is seriously stressed by A1 due to the rapid spread of acid soil,but the reason of root growth inhibition is still unknown.This study aimed to understand the mechanism of root growth inhibition under A1 stress.We investigated the parameters relative to oxidative stress,including content of aluminium,hydrogen peroxide (H2O2),malondialdehyde (MDA),and those involved in defense responses such as oxidative stress activity of peroxidase and content of lignin.The result showed that all values,including content of A1,H2O2,MDA,content of lignin,activity of peroxidase,increased in both maize lines after Al treatment.Notably,after Al exposure for 24 h,the changes of the physiological parameters were in maximum.Meanwhile,lignin content and the activity of peroxidase were increased,up to the peak value of 0.126 OD and 2.04 U,respectively,in the A1 tolerant 178,much higher than that of the A1 sensitive Mo17.On the contrary,the maximum content of A1,H2O2 and MDA in Mo17 was 1.835 μg g-1,16.71 μrnol g-1,40.2 nmol g-1,respectively,after Al treatment,much higher than that in 178.These results suggested that the inhibition of maize root growth is correlated with the Al-induced lipid peroxidation,and that the changes oflignin content and activity ofperoxidase may be a defense response caused by A1 toxicity in maize.
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万方数据
维普
