Mechanism of antagonistic bacteria Pseudomonas putida 1A00316 from the South Pole soil against Meloidogyne incongnita
Meloidogyne incongnita causes serious damage to crops.Nowadays,attention has been paid to the side effects of chemical insecticides control.It is important to isolate the antagonistic microbe against root knot nemtaode from microbial resources.In this study 1 432 marine and polar isolates were screened against nematode to identify 9 strains with high nematocidal activity to study its biocontrol effect on nematode by pot experiments.Among them the strain 1A00316 isolated from the South Pole showed good inhibition on M.incongnita in vitro and in pot experiment,with biocontrol efficiency on nematodes as 71.67％.The experiment of induced systemic resistance (ISR) was conducted to study the possible mechanisms of 1A00316 against M.incongnita by determining the enzymes.Strain identification showed the strain 1A00316 belonged to Pseudomonas putida.The ISR mediated by strain 1A00316 in tomato clearly showed an improvement of activity of three defense enzymes:phenylalnineammonialyase (PAL),polyphenol oxidase (PPO) and peroxidase (POD) in tomato plants.The result indicated strain 1A00316 itself could induce systematic resistance to tomato and enhance their resistance against nematode.This research showed that P.putida strain 1A00316 has good resistance to M.incongnita,of both direct and induced systemic resistance,therefore can be used as microbial agents.
NETL
NSTL
万方数据
维普
