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南方灵芝子实体的化学成分

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南方灵芝[Ganodermaaustrale (Fr.) Pat.,Bull.]为一种常见的生长在阔叶树枯立木、倒木和伐桩上的灵芝属真菌,具有很好的药用和保健价值.采用硅胶、凝胶柱色谱、反相ODS柱和高效液相色谱等方法从野生南方灵芝子实体的乙醇提取物中分离到14个化合物,结合核磁共振(1H-NMR,13C-NMR)、质谱(MS)、红外(IR)、紫外(UV)等波谱技术将其分别鉴定为灵芝烯酸G甲酯(Methyl ganoderenate G,1)、灵芝烯酸G(Ganoderenic acid G,2)、灵芝烯酸A (Ganoderenic acid A,3)、灵芝烯酸A甲酯(Methyl ganoderenate A,4)、灵芝烯酸D(Ganoderenic acid D,5)、灵芝烯酸F (Ganoderenic acid F,6)、灵芝酸AP (Ganoderic acid AP,7)、7β,23α-dihydroxy-3,11,15-trioxolanosta-8,20E(22)-dien-26-oic acid (8)、Elfvingic acid A (9)、3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (10)、对羟基苯甲酸(p-hydroxybenzoic acid,11)、3-羟基-4-甲氧基苯甲酸(3-hydroxy-4-methoxybenzoic acid,12)、油酸-α-单甘油酯(9-octadecenoic acid-2,3-dihydroxypropyl ester,13)及谷甾醇(β-sitosterol,14).其中,化合物1为新化合物,化合物1-9为从南方灵芝中首次分离得到的羊毛甾烷型三萜化合物.
Chemical constituents of the fruiting bodies of Ganoderma australe
This research aimed to study the chemical constituents of fruiting bodies of wild Ganoderma australe (Fr.) Pat.,Bull.of the Ganoderma P.Karst.genus.Compounds 1-14 were isolated by the combination of repeated silica gel chromatography,Sephadex LH-20,reversed-phase ODS column,and high performance liquid chromatography.The structures of the compounds were elucidated on the basis of nuclear magnetic resonance spectroscopy,mass spectrometry,IR,and UV spectra.As a result,fourteen compounds,including a new compound (1),were isolated and elucidated as Methyl ganoderenate G (1),Ganoderenic acid G (2),Ganoderenic acid A (3),Methyl ganoderenate A (4),Ganoderenic acid D (5),Ganoderenic acid F (6),Ganoderic acid AP (7),7β,23α-dihydroxy-3,11,15-trioxolanosta-8,20E(22)-dien-26-oic acid (8),Elfvingic acid A(9),3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(10),p-hydroxybenzoic acid (11),3-hydroxy-4-methoxybenzoic acid (12),9-octadecenoic acid,2,3-dihydroxypropyl ester (13),and β-sitosterol (14).This research isolated nine lanostane-type triterpenoids (Compounds 1-9) from G.australe for the first time.

Ganoderma australefruiting bodychemical constituentstriterpenoidssterolsMethyl ganoderenate G

张春杰、杨婷、李国友、袁小红、马泽刚

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西南科技大学生命科学与工程学院 绵阳621000

中国科学院成都生物研究所 成都610041

南方灵芝 子实体 化学成分 三萜 甾醇 灵芝烯酸G甲酯

国家自然科学基金四川省生物质资源利用与改性工程技术研究中心开放基金

2127218912zxsk03

2015

应用与环境生物学报
中国科学院成都生物研究所

应用与环境生物学报

CSTPCDCSCD北大核心
影响因子:0.972
ISSN:1006-687X
年,卷(期):2015.21(2)
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