Screening, colonization and application evaluation of biocontrol bacteria against tobacco black shank disease
Tobacco black shank disease is a serious soil-spread fungal disease,leading to great economic loss to tobacco crop of China.To prevent and control tobacco black shank disease,three strains (ZH,C9 and C20) with high antagonist ability against tobacco black shank pathogens (Phytophthora parasitica var.nicotianae) were isolated.By physiological,biochemical test and gene sequence analysis,the three strains were identified as Bacillus amyloliquefaciens.Colonization experiment results showed that the B.amyloliquefaciens ZH had high colonization ability in the plant and soil.After 123 d,the bio-control bacteria number reached 9.07 × 106 CFU/g and 1.2 × 107 CFU/g,respectively,in the rhizosphere soil and rhizoplane soil,and 1.83 × 104CFU/g in tobacco stem and 5.58 × 104 CFU/g in the leaf.Bio-control bacteria field experiment with normal use of chemical pesticides as control found the biocontrol effect of the treatment with mixed-strain fermentation liquor ranging from 85.37％ to 95.11％ during different periods.At the same time,the tobacco yield was increased by 20.18％,with a 31.27％ increase in production value.The results suggested that the bio-control bacteria are effective against tobacco black shank disease,therefore of large-scale agricultural application potential.
tobacco black shank diseasebiological controlBacillus amyloliquefacienscolonization
NETL
NSTL
万方数据
维普
