Methane metabolic microbial community structure in the active layer and the permafrost layer of the Qilian permafrost, China
Methane metabolic microbial community in the permafrost soil can produce or oxidize methane,which affects processes of permafrost methane-cycling,therefore has an important role in the global greenhouse release and regulation.At present,research of methane metabolic microbial community structure in the soil of permafrost has received extensive attention.In order to understand the distribution and diversity of methane metabolic microbes in the permafrost,we studied the methanogen and methanotroph community compositions in the active layer and the permafrost layer of soils in Qilian Mountain,China.The molecular biology methods were used to amplify the methanogen mcrA genes and the methanotroph pmoA genes,then their gene clone libraries constructed.Phylogenetic analysis of the sequences based on sequence similarity was carried out and the community diversity was calculated.The retrieved methanogen mcrA genes sequences were classified into five lineages.The predominant groups of the active layer belonged to Rice cluster I,Methanosarcinaceae,Methanomicrobiales,Methanosaetaceae and Methanobacteriaceae,and those in the permafrost layer included Rice cluster I,Methanosarcinaceae and Methanobacteriaceae.All the methanotrophpmoA genes sequences were affiliated to the same phylum Proteobacteria.In the active layer,Proteobaeteria was classified into two lineages including Methylocystis (α-Proteobaeteria,type Ⅱ) and Methylobacter (γ-Proteobacteria,type Ⅰ).In the permafrost layer,Methylocystis was the predominant lineage.The results indicated that there existed a certain difference in methane metabolic microbial composition between the active layer and the permafrost layer of soils in Qilian Mountain.
Qilian Mountainpermafrostmicrobial community structuremethanogensmethanotrophs
NETL
NSTL
万方数据
维普
