Isolation and screening of cellulose decomposing microbe and the straw decomposing effect of complex microbial system
This study aimed to isolate cellulose decomposing microbes from the soil under Rhododendron sp.in Gongga Mountain and to construct a complex microbial system with high cellulolytic ability.All together 79 strains were isolated using two pretreatment approaches.Congo red medium and DNS method were employed to evaluate their carboxymethyl cellulase activity.The results showed 15 strains having a great CMCase ability.Subsequently,the cellulose degradation ability of the 15 strains was determined by analysis results of filter paper degradation,corn straw degradation and degradation rate of corn straw.Based on the antagonism effect among strains,the isolates with high efficiency in cellulose decomposing ability and no antagonism were selected to construct five complex microbial systems:A (112,146,156,171),B (145,147,150,153),C (110,116,174),D (147,154,171),and E (145,14,150,152,153),all of which were further measured the filter paper enzyme activity and corn straw degradation rate.The results showed that the corn straw degradation rate of community C was 50.71％ higher than that of 116 single strain belonging to community C,and that the corn straw degradation rate of strains community D was 41.54％ higher than that of 154 single strain belonging to community D.The 16S rRNA gene sequence similarity between the six isolates of two communities and the typical strains published in Genbank was 99％ to 100％,and those sequences belonged to four genera Paenibacillus sp.,Bacillus sp.,Acinetobacter sp.and Streptomyces sp.Our research revealed that the cellulose decomposing ability of complex microbial system is higher than that of single strain.Both Complex C and D showed a great capacity in cellulose decomposing,therefore of great exploitation value.
cellulose decompositionseparation and identificationcomplex microbial systemfilter paper degradationcorn straw
NETL
NSTL
万方数据
维普
