Diversity and succession of bacteria during the fermentation of a cigar wrapper using high throughput sequencing technology and traditional isolation
Bacteria play an important role in the process of cigar fermentation. It is important to study the succession of the bacterial community during cigar fermentation to understand the mechanism of tobacco fermentation. This experiment analyzed the community structure of surface bacteria, species richness, and the succession of bacteria on a Mexican cigar wrapper at different fermentation stages using Illumina Mi Seq high-throughput sequencing technology (HTS) and traditional isolation. The HTS obtained 514 641 effective sequences, including 65 operational taxonomic units (OTUs). The main microbial species included Corynebacterium sp., Staphylococcus sp., Acinetobacter sp., Pseudomonas sp., and Bacillus sp. The results of the HTS showed an abundant surface bacterial community, and the structure of the community changed with the fermentation process. The dominant microorganism genera were Corynebacterium (44.64％), Pseudomonas (40.88％), and Staphylococcus (72.78％). The FJQY-1 and FJQY-2 bacterial communities had higher alpha diversity richness than that of the other samples. The FJQY-2, FJQY-3, and FJQY-4 samples had similar beta diversity values that were high. Traditional isolation showed that the dominant bacteria during fermentation were Bacillus sp. (55.10％) and Staphylococcus sp. (22.45％), which does not comprehensively reflect the bacterial community and succession on the cigar wrapper at different periods. There was succession of the bacterial community during the fermentation process. The HTS plays a vital role in revealing the succession of the bacterial community. Traditional isolation and identification cannot fully ref lect the diversity and succession of the bacterial community, however, it can be helpful in future research on microbial function during the fermentation process.
cigar wrapperhigh throughout sequencingfermentation stagebacteria diversitycommunity succession
NETL
NSTL
万方数据
维普
