Identification and regulation of the HIPPs/HPPs gene family from the Polygonatum cyrtonema Hua transcriptome
PcHIPPs/PcHPPs are a class of intracellular soluble metal-binding proteins that play important roles in regulating plant heavy metal homeostasis,detoxification mechanisms,and heavy metal tolerance in plants.The HIPPs/HPPs gene family has not been studied in Polygonatum cyrtonema Hua.Based on different transcriptome data of P.cyrtonema Hua,the physical and chemical properties,subcellular localization,conserved motifs,and phylogenetic tree were analyzed by bioinformatics and their functions were preliminarily analyzed to study the expression of HIPPs/HPPs genes under different lead(Pb)treatments.The results showed that 68 PcHIPPs and 43 PcHPPs genes were identified from the P.cyrtonema Hua.The amino acid number of the PcHIPPs gene family is 134-501,the molecular weight is 15.53-53.09 ku,and the isoelectric point is 5.12-9.88.The amino acid number of the PcHPPs gene was 101-352,the molecular weight was 11.46-38.23 ku,and the isoelectric point was 5.58-10.41.Subcellular localization prediction showed that 73 PcHIPPs/PcHPPs genes were distributed in the nucleus.KEGG analysis showed that the PcHPP3,PcHIPP28-5,PcHIPP28-6,and PcHPP1-5 genes were involved in the metabolic process.The PcHIPPs/PcHPPs genes played a major role in the rhizosperms of P.cyrtonema Hua,and most of the PcHIPPsIPcHPPs genes showed evident downregulation after 48 hours of salt stress treatment.The shriveled and yellowed leaves of P.cyrtonema Hua tissue-culture seedlings gradually increased in the Pb treatment,and low concentration of Pb stress promoted the chlorophyll content of plants.In contrast,a high concentration of Pb stress inhibited the chlorophyll content of plants.In addition,the expression of PcHIPPsIPcHPPs genes initially increased and then decreased with the increase of Pb concentration.This study suggests that PcHIPPs/PcHPPs genes play an important regulatory role in the P.cyrtonema Hua Pb stress response,laying a foundation for the function of PcHIPPs/PcHPPs genes in response to heavy metal stress.
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