Ecological effect of Enterobacter ludwigii A18 dispersal along hyphae of Fusarium sp.JK
Migration of bacterial cells along fungal hyphae in soil can broaden bacterial niches and enhance their ecological function.However,the ecological effect of bacterial dispersal along phytopathogenic fungi needs further investigation.In this study,the ability of attachment and dispersal of Enterobacter ludwigii A18 along Fusarium sp.JK hyphae were investigated via a co-culture system,while the growth and sporulation of Fusarium sp.JK in the presence of E.ludwigii A18 was also explored.The results indicated that dispersion of E.ludwigii A18 was present in a semi-solid agar medium(3 g/L agar)and was absent when the agar concentration was higher than 5 g/L.However,E.ludwigii A18 could migrate along the fungal hyphae of Fusarium sp.JK,with the biomass reaching 108 CFU/g agar at the migration site.In the presence or absence of E.ludwigii A18,no significant difference was observed in the fungal growth rates.On the other hand,fungus sporulation was significantly suppressed at a 90％inhibition rate when Fusarium sp.JK was inoculated with E.ludwigii A18,compared to that in fungal monoculture.Bacterial biofilm formation on the surface of fungal hyphae was also confirmed by scanning electron microscopy.Additionally,fungal exudates could support the growth of E.ludwigii A18,with bacterial biomass increased from 10607±009 CFU/mL to 10907±0.10 CFU/mL.In conclusion,our data demonstrated that E.ludwigiiA18 could disperse along fungal hyphae of Fusarium sp.JK,utilize fungal exudate and broaden bacterial niche.Additionally,E.ludwigii A18 could suppress the spore formation of Fusarium sp.JK,thus alleviating damage of phytopathogenic fungus to crops.
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