Abundance and community structure of methane-oxidizing microorganisms in riparian reed wetland soil of Dianchi Lake
Riparian wetlands are an important source of methane emissions,and methane oxidation,driven by methane-oxidizing microorganisms,plays an important role in regulating methane emissions from wetlands and alleviating the greenhouse effect.To explore the abundance and community structure of methane-oxidizing microorganisms in the riparian reed wetland soil of Dianchi Lake,the soils of five typical reed wetlands along Dianchi Lake(Xihua Wetland,Daqing River,Laoyu River,Cixiang River,and Baiyu River)were used as the research objects.Quantitative fluorescence PCR and high-throughput sequencing were used to study the gene abundance and community composition of methane-oxidizing microorganisms in typical riparian wetlands and to explore the effects of the physical and chemical properties of soil and overlying water.The results showed that the abundances of pmoA,ANME-2d,and NC10 genes in the riparian reed wetlands of Dianchi Lake were 1.34 × 107-5.45 × 107 copies/g,9.92 × 104-8.90 × 105 copies/g and 9.54 × 105-6.85 × 109 copies/g,respectively.Significant differences were observed in the abundances of methane-oxidizing microorganisms in the different riparian reed wetlands of Dianchi Lake.The abundance of pmoA gene was the highest in the Xihua Wetland(P＜0.01),while those of ANME-2d and NC10 were the highest in the Cixiang(P＜0.01)and Laoyu Wetlands(P＜0.001),respectively.The major groups of methane-oxidizing microorganisms in the riparian reed wetlands of Dianchi Lake were type Ⅱ aerobic methane-oxidizing bacteria and denitrifying anaerobic methane-oxidizing microorganisms(ANME-2d archaea and NC10 bacteria).Correlation and redundancy analyses showed that the key environmental factors affecting methane-oxidizing microorganisms in the riparian reed wetland of Dianchi Lake included soil total phosphorus,soil total nitrogen,overlying water total phosphorus,and overlying water nitrate-nitrogen.The eutrophication status of Dianchi Lake has an important effect on the abundance and community distribution of methane-oxidizing microorganisms in riparian wetlands.
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