Effects of reclaimed water irrigation on soil microbial community composition in urban green space
Reclaimed water(RW)is a sustainable alternative water resource that can alleviate the global water supply-demand imbalance.The successional characteristics of urban greenspace soil microbial communities in response to reclaimed water irrigation were explored using Illumina MiSeq high-throughput sequencing to reveal the effects of tap,reclaimed,and wastewater irrigation on the diversity and composition of soil bacterial and fungal communities in urban green spaces.The results showed that the dominant bacteria(relative abundance＞5％)in the three types of irrigated soils were Actinobacteria,Proteobacteria,Firmicutes,Chloroflexi,Bacteroidota,and Acidobacteriota,and the dominant fungi were Ascomycota and Basidiomycota.Analysis of variance showed that reclaimed water irrigation did not cause significant changes in α-diversity and overall community structure of soil bacterial and fungal communities.However,it promoted the growth of Actinobacteria,Proteobacteria,Acidobacteriota,and Basidiomycota(relative abundances increased by 6.58％,5.85％,3.95％,and 21.70％,respectively),while inhibiting the growth of Firmicutes,Bacteroidota,and Ascomycota(relative abundances decreased by 12.38％,3.24％,and 3.86％,respectively).This may be related to changes in soil pH,dissolved organic carbon(DOC),and ammonia nitrogen content.Differential zero-radius operational taxonomic unit(zOTU)clustering analysis showed that the changes in soil bacterial and fungal community composition caused by reclaimed water irrigation were more similar to those caused by wastewater irrigation.The effects of reclaimed water irrigation on soil microbial community composition were closer to those of wastewater irrigation compared with tap water irrigation.Thus,the quality of reclaimed water should be strictly controlled when used for urban green space irrigation to minimize its potential accumulative effect.
reclaimed water irrigationurban green spacehigh-throughput sequencingsoil microbial community
万方数据
维普
