β-Carotene production by solid-state fermentation of Neurospora crassa and the effects of feeding it to laying hens
Neurospora crassa can produce β-carotene through solid-state fermentation utilizing agricultural by-products.To date research into feeding animals such probiotic fermentation products is lacking.We applied one-way and orthogonal experiments to optimize parameters for β-carotene production during N.crassa fermentation using soybean dregs as substrate.A shallow tray test was conducted as a scale-up experiment using the elucidated optimal fermentation conditions.Finally,the fermentation products directly replaced soybean meal in the diet of laying hens.The optimum conditions to produce β-carotene by solid state fermentation using soy bean dregs as a substrate were:30 ℃ fermentation temperature,60％substrate moisture content,substrate pH of 6,and 72 h fermentation period.The yield of β-carotene reached 202.2 μg/g(fermentation products)in orthogonal testing,and the yield of β-carotene was 210 μg/g(fermentation products)in the platter scale-up fermentation.In the laying hen feeding experiment,replacement of soybean meal with fermentation products increased the laying rate of laying hens from 75.25％to 85.75％,significantly reduced the feed-to-egg ratio,and increased daily feed intake and weight gain.In addition,the color and β-carotene content of eggs were also significantly increased.Direct replacement of soybean meal with N.crassa fermentation products could simplify fermented feed production,significantly improve the growth and production performance of laying hens,and enable the accumulation of β-carotene in eggs,resulting in a significant improvement in color.This study provides an important reference for using N.crassa to produce animal feed rich in β-carotene.
Neurospora crassasolid-state fermentationβ-carotenelaying hen
万方数据
维普
