Impact of earthworm inoculation on arbuscular mycorrhizal fungal diversity and pepper growth in microplastics-contaminated vegetable soil
Modern intensive planting has led to increasing soil microplastic(MP)contamination in vegetable fields.Arbuscular mycorrhizal(AM)fungi are known to enhance plant nutrient uptake and resistance.This study investigates the impact of earthworm inoculation on the diversity and functionality of indigenous AM fungi in soil.A pot experiment was conducted using soil amended with 0.1％polyethylene terephthalate(PET).Inoculation with earthworms(Eisenia fetida)significantly reduced soil organic carbon and available phosphorus concentrations by 8.3％and 20.3％,respectively.It also tended to reduce species richness and diversity indices of indigenous AM fungi,shifting the dominant genus from Glomus(51.9％relative abundance)to Paraglomus(45.6％relative abundance).Additionally,earthworm inoculation significantly increased the population size and colonization rate of AM fungi,as well as the biomass and fruit yield of pepper(Capsicum annuum L.)by 47％to 79％.Redundancy and correlation analyses revealed a significant positive correlation between pepper fruit yield and shoot biomass,with shoot biomass positively correlated with AM fungal population size,colonization rate,and soil alkaline phosphatase activity.However,AM fungal diversity indices were positively correlated with soil organic carbon concentration.Earthworm inoculation may selectively filter the soil AM fungal community species composition,reducing the diversity indices while ingesting and accumulating MPs,enhancing soil respiration,and decreasing soil organic carbon concentration.In conclusion,earthworm inoculation can promote AM fungal population propagation and colonization by decreasing diversity indices and enhancing their growth-promoting function in MPs(i.e.,PET)-contaminated vegetable fields.
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