Effects of arbuscular mycorrhizal fungus and copper application on plant growth and cadmium uptake in Chinese milk vetch(Astragalus sinicus L.)
Plant-microbial interactions play a critical role in alleviating abiotic stress and enhancing crop productivity.However,there are limited reports on the induction of plant copper(Cu)uptake by arbuscular mycorrhizal fungi(AMF)and the subsequent reduction in cadmium(Cd)uptake.In this study,we conducted a pot experiment with four treatments:control(CK),AMF inoculation(AM),Cu addition(Cu),and AM+Cu,with and without Cd addition.The aim was to explore the effects of AMF inoculation and exogenous Cu addition on the growth,absorption,and transport of Cd in Astragalus sinicus L.under Cd stress.Results showed that AMF inoculation and Cu supplementation significantly promoted root development,plant biomass,soluble sugar and proline content,and activities of peroxidase and superoxide dismutase.The contents of hydrogen peroxide and Cd in aboveground parts and the transfer of Cd from roots to shoots were reduced in the AMF-and Cu-treated plants.The combined treatment had a greater promoting effect than the separate treatments.This study concluded that Cu and AMF are beneficial for the growth of A.sinicus under Cd stress and mitigate metal pollution damage by reducing active oxygen molecules,increasing osmolytes content,and enhancing antioxidant enzyme activity,thereby reducing Cd ion accumulation in shoots.Cu enhances the symbiotic effect,potentially related to mycorrhiza-mediated enhanced Cu absorption and resulting metal tolerance.This study provides a further understanding of the mechanisms of heavy metal remediation by AMF.
arbuscular mycorrhizal fungiheavy metal pollutionplant and microbial remediationAstragalus sinicus L
NETL
NSTL
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