Identification of miRNAs related to high-temperature stress in rice at the booting stage and prediction of their target genes
Rice is an economically important food crop worldwide.The emergence and expansion of global warming present an urgent problem in rice-planting areas,as high temperatures can cause significant plant damage.Previous studies have shown that microRNAs(miRNAs)and their target genes play crucial roles in regulating plant growth and development.This study used high-throughput sequencing technology to construct small RNA libraries of rice 9311 at the booting stage after heat treatment for 0,3,6,and 12 hours.Bioinformatics analysis identified 464 known miRNAs and 123 novel miRNAs in rice,with 25 differentially expressed miRNAs—12 upregulated and 13 downregulated.Additionally,253 corresponding target genes were predicted.Gene Ontology(GO)analysis revealed that the target genes were highly enriched in transcription regulation,integral components of membranes,and ATP-binding items related to biological processes,cell components,and molecular functions.KEGG pathway analysis indicated that 13 target genes were enriched in metabolic pathways interacting with eight differentially expressed miRNAs to form a regulatory network.Further analysis using qRT-PCR of eight differentially expressed miRNAs and their target genes in response to high-temperature stress showed that osa-miR164f,osa-miR1850.1,osa-miR6249a,and novel_miR_85 had negative regulatory relationships with their target genes,whereas osa-miR2871a-5p and novel_miR_87 had positive regulatory relationships.Based on a comprehensive analysis,miRNAs in rice respond to high-temperature stress by targeting gene expression.This study identified relevant miRNAs and candidate genes crucial for understanding the regulatory mechanisms of high-temperature stress in rice.
Oryza sativahigh temperature stressmiRNA,target geneexpression regulation
NETL
NSTL
万方数据
