Genome-wide identification and expression of the DXS gene family in Huangdan(Camellia sinensis)
1-Deoxy-D-xylulose-5-phosphate synthetase(DXS)is the first rate-limiting enzyme in the methylerythritol-4-phosphate(MEP)pathway of plant terpene synthesis,playing a crucial role in tea plant resistance.To investigate the potential functions of the DXS gene family,we employed bioinformatics analysis and quantitative reverse transcription PCR(qRT-PCR)to identify and analyze the expression patterns of the CsDXS gene family.Five DXS gene family members were identified from Huangdan and five from Tieguanyin tea plants,named HD-CsDXS1-5 and TGY-CsDXS1-5,respectively.Phylogenetic analysis categorized them into three subfamilies,with consistent exon-intron structure within each subfamily.Promoter analysis revealed numerous cis-acting elements associated with stress responses,hormones,and growth.Quantitative fluorescence detection indicated significant upregulation of HD-CsDXS2 and HD-CsDXS5 under methyl jasmonate(MeJA),gibberellin(GA),abscisic acid(ABA),low temperature,and drought treatments;HD-CsDXS1 and HD-CsDXS4 were significantly upregulated under GA,ABA,and drought treatments;and HD-CsDXS3 was upregulated only under GA and drought treatments.These findings suggest that CsDXS genes are induced under abiotic stress and may contribute to the stress resistance of tea plants.Our results provide a foundation for further research on the functions of the DXS gene family in tea plants.
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